Composite

Part:BBa_K316007:Design

Designed by: IC 2010 Team   Group: iGEM10_Imperial_College_London   (2010-10-21)


Recombinant GFP-XylE protein under Pveg promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1229
    Illegal NgoMIV site found at 1401
    Illegal AgeI site found at 1752
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 785


Design Notes

Standard biobrick cloning of intermediary parts


Source

Pveg and spoVG biobricks BBa_K143053 added to 5'his tagged-GFP-XylE construct BBa_K316004.

References